Our local DOT drug and alcohol testing centers are located in Cowan CA and the surrounding areas providing DOT drug testing, DOT alcohol testing and DOT physicals for all DOT modes regulated by Part 40. Same day service is available at our Cowan CA DOT drug testing facilities and most of our DOT drug testing locations are within minutes of your home or office.
What type of DOT Testing is required?
Coastal Drug Testing provides DOT pre-employment, random, post-accident, reasonable suspicion and return to duty testing at our Cowan CA DOT drug testing centers.
If you hold a CDL license, a large, medium or a small trucking company, Coastal Drug Testing has a complete DOT compliance package which includes all the requirements to comply with CFR 49 part 40.
All Coastal Drug Testing DOT drug testing centers utilize SAMHSA Certified laboratories and a licensed Medical Review Officer as required by DOT part 40 regulations.
The U.S Department of Transportation (DOT) requires that all DOT regulated "safety sensitive" employees have a negative DOT pre-employment drug test result on file and be actively enrolled in a DOT approved random drug and alcohol random testing pool (consortium).
In addition, if a DOT regulated company has more than one "safety sensitive" employee, the employer must also have a written DOT drug and alcohol policy along with an on-site supervisor that must have completed a reasonable suspicion supervisor training program.
On the road and need a DOT Drug or Alcohol test? No Worries!
To be compliant with DOT regulations, a company's DOT drug and alcohol testing program must have the following components:
- Employee Drug Testing
- Written Drug and Alcohol Policy
- Supervisor Training
- Substance Abuse Referral
- Employee Education
- Random Selection Program
- Post Accident Testing
- Designated Employer Representative
- Federal Chain of Custody Forms
- Part 40 Regulations on File
The Department of Transportation (DOT) has specific drug and alcohol testing requirements for the all transportation modes all DOT agencies.
Our modes included are:
- Federal Motor Carrier Safety Administration (FMCSA)
- Federal Aviation Administration (FAA)
- Federal Transit Administration (FTA)
- United States Coast Guard (USCG)
- Pipeline and Hazardous Materials Safety Administration (PHMSA)
- Federal Railroad Administration (FRA)
Are You Enrolled in a DOT Consortium?
Individuals who are employed in a position designated as "safety sensitive" must be actively enrolled in a random drug and alcohol testing program. Oftentimes, covered employees will join a group of other DOT regulated employees in a random testing program and this is referred to as a DOT Consortium. Generally, an employer who has less than fifty employees or single operators will join the consortium which will comply with the random drug and alcohol testing requirements of 49 CFR Part 40. Employers that have over 50 employees who are regulated by Part 40 may elect to be enrolled in a "stand alone" random testing pool.
The DOT consortium is cost effective and complies with all requirements of 49 CFR Part 40 which mandates that all "safety sensitive" employees be enrolled in a random drug and alcohol testing program.
The Department of Transportation (DOT) has strict regulations requiring regulated companies and independent operators (CDL License Holders) to be an active member of a DOT drug and alcohol Consortium and failure to comply with these regulations can result in significant fines and other DOT sanctions.
We are fully versed in the DOT procedures for pre-employment drug testing, random drug testing, reasonable suspicion drug testing, post-accident drug testing, return to duty drug testing and follow up drug testing.
DOT regulated companies with multiple safety sensitive employees must also have an employee within the company who is assigned as the "designated employer representative" (DER). This is the person responsible for removing any DOT "safety sensitive" employee who is covered by 49 CFR Part 40 from performing a DOT safety sensitive position when a positive drug or alcohol test result has occurred or an employee has refused to take a required DOT test.
If you have recently become a DOT regulated company, within the next 18 months the Department of Transportation (DOT) will conduct a "new entrant" inspection to ensure that you are in compliance with all DOT regulations including the drug and alcohol testing requirements. If you are currently a DOT regulated company, you are subject to regular inspections to ensure compliance.
Avoid DOT fines, penalties and be complaint with all DOT drug and alcohol testing regulations! Coastal Drug Testing can assist small, medium and large DOT companies in complying with all requirements of 49 CFR Part 40.
DOT Drug Testing Locations in Cowan CA
(Don't see a location near you? Call us (800) 828-7086)
Local Area Info: H3K27me3
H3K27me3 is a histone methylation occurring on the amino (N) terminal tail of the core histone H3. This tri-methylation is associated with the downregulation of nearby genes via the formation of heterochromatic regions.
The genomic DNA of eukaryotic cells is wrapped around special protein molecules known as Histones. The complexes formed by the looping of the DNA are known as Chromatin. The basic structural unit of chromatin is the Nucleosome: this consists of the core octamer of histones (H2A, H2B, H3 and H4) as well as a linker histone and about 180 base pairs of DNA. These core histones are rich in lysine and arginine residues. The carboxyl (C) terminal end of these histones contribute to histone-histone interactions, as well as histone-DNA interactions. The amino (N) terminal charged tails are the site of the post-translational modifications, such as the one seen in H3K27me3.
The placement of a repressive mark on lysine 27 requires the recruitment of chromatin regulators by transcription factors. These modifiers are either histone modification complexes which covalently modify the histones to move around the nucleosomes and open the chromatin, or chromatin remodelling complexes which involve movement of the nucleosomes without directly modifying them. These histone marks can serve as docking sites of other co-activators as seen with H3K27me3. This occurs through polycomb mediated gene silencing via histone methylation and chromodomain interactions. A polycomb repressive complex (PRC); PRC2, mediates the tri-methylation of histone 3 on lysine 27 through histone methyl transferase activity. This mark can recruit PRC1 which will bind and contribute to the compaction of the chromatin.